A pdf of the Tier 2 Application can be found here: https://units.fisheries.org/fhs/wp-content/uploads/sites/30/2020/07/Tier-II-application-07-01-20.pdf

Provide an updated copy of your laboratory manual, including a description of any specific operational changes associated with standard procedures.

Provide two years of equipment calibration and standardization records (e.g. calibration invoices, freezer/incubator records, etc.) that have been collected since receiving Tier I approval.

Provide updated CV and qualifications of all employees.

Provide a BSL-2 compliance letter from a qualified inspector associated with entities such as USDA/APHIS, Veterinary Medical Officer, state or university safety officer or other qualified individuals that have been pre-approved by the QA/QC Committee.